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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which leads to chromosome instability, and therefore may well contribute to tumorigenesis. This protein was located to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant job in promoting cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are described. Two further splice variants were claimed but thorough nucleotide sequence information will not be but out there.
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Blocking peptides are peptides that bind specially towards the goal antibody and block antibody binding. These peptide generally consists of the epitope identified via the antibody.
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This guideline outlines more probable optimization of mobile and immunoassay parameters to make certain the absolute best final results are...
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This guidebook outlines additional feasible optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
Antibodies certain to the blocking peptide not bind on the epitope around the focus on protein. By evaluating the staining within the blocked antibody versus the antibody on your own, one can see which staining is distinct.
Focus on Facts Cyclin E belongs to the extremely conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance with the mobile cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation patterns which lead into the temporal coordination of each mitotic function. Cyclin E sorts a complex with and capabilities as a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates on the G1-S stage boundary which is degraded as cells development by way of S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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